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Neurogenesis Laboratory

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Neurogenesis Laboratory


About the NL

The Neurogenesis Laboratory (NL) is interested in the study of adult neurogenesis and brain repair. Using both in vitro and in vivo approaches, the NL group is investigating the mechanisms that regulate neural stem cell proliferation and differentiation with particular emphasis directed towards several disorders including neuroinflammatory conditions, such as epilepsy, stroke or neurodegenerative disorders. The NL unit is particularly interested in understanding the mechanisms of how post-injury neurogenesis occurs in the lesion micro-environment and in developing new ways to achieve efficient endogenous neurogenesis and/or successful survival and maturation of grafted new neurons.


Publications (2010-2018)

Pinheiro G, Prata D, Araújo IM, Tiscornia G (2018) The African Spiny Mouse (Acomys spp.) as an emerging model for development and regeneration. Laboratory Animals, 1:23677218769921.

Santos AI, Carreira BP, Izquierdo-Álvarez A, Ramos E, Lourenço AS, Santos D, Morte MI, Ribeiro LF, Marreiros A, Sánchez-López N, Marina A, Carvalho CM, Martínez-Ruiz A, AraújoIM (2018) S-nitrosylation of Ras mediates nitric oxide dependent post-injury neurogenesis in a seizure model.Antioxidants and Redox Signaling. 28(1):15-30.

Mestre AL, Cerquido M, Inácio PMC, Asgarifar S, Lourenço AS, Cristiano ML, Aguiar P, Medeiros MCR, Araújo IM, Ventura J, Gomes HL (2017) Ultrasensitive gold micro-structured electrodes enabling the detection of extra-cellular long-lasting potentials in astrocytes populations. Scientific Reports, 7(1):14284.

Mestre AL, Inácio PMC, Elamine Y, Asgarifar S, Lourenço AS, Cristiano MD, Aguiar PD, Medeiros MCR, Araújo IM, Ventura J, Gomes HL (2017) Extracellular electrophysiological measurements of cooperative signals in astrocytes populations. Frontiers in Neural Circuits, 11:80.

Machado VM, Lourenço AS, Florindo C, Fernandes R, Carvalho CM, Araújo IM CA (2017) Overexpression of calpastatin preserves cognitive function following seizures, while maintaining post-injury neurogenesis. Frontiers in Molecular Neuroscience 10:60.

Rochar PRF, Medeiros MCR, Kintzel U, Vogt J, Araújo IM, Mestre A, Mailänder V, Schlett P, Dröge M, Schneider L, Biscarini F, de Leeuw DM, Gomes HL (2016) Extracellular electrical recording of pH-triggered bursts in C6 glioma cell populations. Science Advances 2(12):e1600516.

Medeiros MCR, Mestre A, Inácio P, Asgarif S, Araújo IM, Hubbard PC, Velez Z, Cancela ML, Rocha PRF, de Leeuw DM, Biscarini F, Gomes HL (2016) An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes. Sensing and Biosensing Research 10:1-8. URL:

Machado NJ, Simões AP, Silva HB, Ardais AP, KAster MP, Garção P, Rodrigues DI, Pochmann D, Santos AI, Araújo IM, Porciúncula OL, Tomé AR, Kofalvi A, Vaugeois J-M, Agostinho P, El Yacoubi M, Cunha RA, Gomes CA (2016) Caffeine reverts memory but not mood impairment in bred-based depressed mice with up-regulated adenosine A2A receptor in hippocampal glutamate synapses. Molecular Neurobiology. URL:

Santos DM, Martins AR, Casanellas I, Ova AB, Araújo IM, Power D, Tiscornia G (2016) Ear wound regeneration in the African Spiny Mouse Acomys cahirinus. Regeneration 3(1):52-61.URL:

Carreira BP*, Santos DF*, Santos AI, Carvalho CM, Araújo IM CA (2015) Nitric oxide regulates neurogenesis in the hippocampus following seizures. Oxidative Medicine and Cellular Longevity 2015:451512. URL:

Carreira BP*, Santos DF*, Santos AI, Carvalho CM, Araújo IM CA (2015) Nitric oxide regulates neurogenesis in the hippocampus following seizures. (Accepted on May 18, 2015, in Oxidative Medicine and Cellular Longevity)

Santos AI, Martinez-Ruiz A, Araújo IM CA (2015) S-nitrosation and neuronal plasticity. British Journal of Pharmacology172(6): 1468-1478.

Machado VM, Morte MI, Carreira BP, Azevedo MM, Takano J, Iwata N, Saido TC, Asmussen H, Horwitz AR Carvalho CM,Araújo IM CA (2015) Involvement of calpains in adult neurogenesis: implications for stroke.Frontiers in Cellular Neuroscience 9:22.

Carreira BP, Morte MI, Santos AI, Lourenço AS, Ambrósio AF, Carvalho CM, Araújo IM CA(2014) Nitric oxide from inflammatory origin impairs neural stem cell proliferation by inhibiting epidermal growth factor receptor signaling. Frontiers in Cellular Neuroscience 8: 343.

Santos AI, Carreira BP, Nobre RJ, Carvalho CM, Araújo IM (2014) Stimulation of neural stem cell proliferation by inhibition of phosphodiesterase 5. Stem Cells International 2014:878397.

Carreira BP, Santos AI, Carvalho CM and Araújo IM (2013) Modulation of adult neurogenesis by the nitric oxide system in Neural Stem Cells. Edited by Intech. Editor: Luca Bonfati. ISBN:980-953-307-863-7

Morte MI, Carreira BP, Falcão MJ, Ambrósio AF, Soares-da-Silva P, Araújo IM, Carvalho CM, (2013) Evaluation of neurotoxic and neuroprotective pathways affected by antiepileptic drugs in cultured hippocampal neurons. Toxicology in vitro 27(8):2193-9202

Morte MI, Carreira BP, Machado VM, Carmo A, Nunes-Correia I, Carvalho CM, Araújo IM(2013) Evaluation of proliferation of neural stem cells in vitro and in vivo. Current Protocols in Stem Cell Biology 2013 Feb; Chapter 2:Unit2D.14.

Martinez-Ruiz A, Araújo IM, Izquierdo-Álvarez A, Hernansanz-Agustín P, Lamas S, Serrador JM (2013) Specificity in S-nitrosylation: a short-range mechanism for NO signaling? Antioxidants and Redox Signaling 19(11):1120-1135.



Project Grants


2018-2020 – Role: Team member. Research project “GT-LightUP – Signaling Gasotransmitters Light Up”. Supported by FCT, Principal Investigator: José Martinho, Instituto Superior Técnico.

2018-2020 – Role: Team Member. Research project “The role of stress granules in Polyglutamine disease: from pathogenesis to molecular therapy”. Supported by FCT, Reference ALG-01-0145-FEDER-29480, Principal Investigator: Clévio Nóbrega, University of Algarve.

2016-2018 – Role: Team Member. Research project “Regeneration in the african spiny mouse”. Supported by FCT, PTDC/BIA-ANM/0697/2014, Principal Investigator: Gustavo Tiscornia.

2016 – 2018 – Role: Team Member. Research project “IMPLANTABLE ORGANIC DEVICES FOR ADVANCED THERAPIES (INNOVATE)”. Supported by FCT, PTDC/EEI-AUT/5442/2014, Principal investigator: Henrique Leonel Gomes.

2013 – 2015 – Role: Principal investigator. Research project “Enhancement of endogenous neurogenesis by nitric oxide: identification of S-nitrosylation targets”. Supported by FCT, PTDC/SAU-OSD/0473/2012.

2011-2014 – Role: Principal investigator. Research project “iCALP – Identification of the physiological roles of calpains in the control of cell proliferation and migration in the central nervous system”. Supported by FCT, PTDC/SAU-NMC/112183/2009.

2012-2013 – Role: Principal investigator. Bilateral cooperative project “Identification of the S-nitrosylation targets of NO in the stimulation of endogenous neurogenesis”. Supported by CRUP, Ação Integrada nº E-10/12.

2010-2013 – Role: Team member. PTDC/SAU-NEU/102612/2008.

2007 – 2010 – Role: Team member. PTDC/SAU-NEU/73119/2006.

2007 – 2010 – Role: Team member.  PTDC/SAU-NEU/81064/2006.



2013 – 2015 – Delegate of the COST action BM1005 – Gasotransmitters: from basic science to therapeutic applications (ENOG: European Network on Gasotransmitters)

2006 – 2010 – FP6-LIFESCIHEALTH-Ref.19063, Network of European Neuroscience Institutes (ENI-NET), Young Investigator.

Science outreach grants:

2014-2018 – Role: Team member. Outreach project for developing an itinerant exhibition for science centres “”Science Trip – Vamos descascar ciência”. Supported by Ciência Viva (150,000 Euro). Consortium of 5 Science Centres belonging to the Ciência Viva network. Coordination: Centro Ciência Viva Lousal.

2011-2013 – Role: Team member.  “Quero mais e melhores células! (Células estaminais: o que são? onde estão? para que servem?)”. Supported by COMPETE – Ciência Viva – 1325.



Representative Images