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Microbiology Research Lab

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Microbiology Research Lab


About the MRL

The Microbiology Research Laboratory (MRL) is focused on investigate bacterial pathogens interactions either in the environment and during their interaction with the host. The potential use of natural compounds to control microbial growth and virulence is also explored.



Publications (2013-2018)

D. Schrama, M.L. Faleiro “The use of two-dimensional electrophoresis to explore Foodborne Bacterial Pathogens responses to gastrointestinal stress”. In: Foodborne Bacterial Pathogens: Methods and Protocols. 2018. Springer International Publishing. A. Bridier (Ed.) DOI 10.1007/978-1-4939-9000-9.

L. Cunha, S. Rodrigues, A.M. Rosa da Costa, M. L. Faleiro, F. Buttini, A. Grenha “Inhalable Fucoidan Microparticles Combining Two Antitubercular Drugs with Potential Application in Pulmonary Tuberculosis Therapy” Polymers 2018,10, pp. 636; doi:10.3390/polym10060636.

S. El-Guendouz, S. Aazza, L. Badiaa, V. Bankova, M. Popova, L. Neto, M. L. Faleiro, M.G. Miguel “Moroccan propolis: a natural antioxidant, anti-bacterial and anti-biofilm against Staphylococcus aureus with no induction of resistance after continuous exposure”. Evidence-Based Complementary and Alternative Medicine (accepted for publication).

M.L. Faleiro, “The Listeria monocytogenes Triad for Success: Food Matrix, Stress Response and Virulence”, Springer International Publishing AG 2017 J.B. Gurtler et al. (eds.), Foodborne Pathogens, Food Microbiology and Food Safety, DOI 10.1007/978-3-319-56836-2_4.

E. Pinto, M. Anselmo, M. Calha, A. Bottrill, I. Duarte, P.W. Andrew, M.L. Faleiro, “The intestinal proteome of diabetic and control children is enriched with different microbial and host proteins”, Microbiology 2017, 163, pp. 161-174. URL:

A.C. Guerreiro, C. M.L. Gago, M.G.C. Miguel, M.L. Faleiro, M.D.C. Antunes, “The effect of edible coatings on the nutritional quality of ‘Bravo de Esmolfe’ fresh-cut apple through shelf-life”, LWT – Food Science and Technology, 2017, 75, pp. 210-219.

M.S. Gomes, M.G. Cardoso, A.C.G. Guimarães, A.C. Guerreiro, C. M.L. Gago, E.V.Vilas Boas, C.M.B. Dias, A.C.C Manhita., M.L. Faleiro, M.G.C. Miguel, M.D.C. Antunes, “Effect of edible coatings with essential oils on the quality of red raspberries over shelf-life”. J Sci Food Agric 2017; 97, pp. 929–938, Doi:10.1002/jsfa.7817

Y. Elamine, S. Aazza, B. Lyoussi, M.D. Antunes, L.M. Estevinho, O. Anjos, M. Resende, M.L. Faleiro, M.G. Miguel, “Preliminary characterization of a Moroccan honey with a predominance of Bupleurum spinosum pollen”. Journal of Apicultural Research January 2017, pp. 1-13.

M.G. Miguel, M.D. Antunes, M.L. Faleiro, “Honey as alternative medicine”, Integrative Medicine Insights, 2017, 12, pp.1–15.

C.I. Silva, S. Aazza, M. L. Faleiro, M.G. Miguel, L. Neto, “The antibacterial, anti-biofilm, anti-inflammatory and virulence inhibition properties of Portuguese honeys”. Journal of Apicultural Research November 2016, 55, pp. 292-304.

S. El-Guendouz, S. Aazza, L. Badiaa, V. Bankova, J.P. Lourenço, A.M.R. Costa, J.F. Mariano, M.G. Miguel, M.L. Faleiro, “Impact of biohybrid magnetite nanoparticles and Moroccan propolis on adherence of methicillin resistant strains of Staphylococcus aureus”. Molecules 2016, 21, 1208; doi:10.3390/molecules21091208.

S. El-Guendouz, S. Aazza, B. Lyoussi, M.D. Antunes, M.L. Faleiro, M.G. Miguel, “Anti-acetylcholinesterase, antidiabetic, anti-inflammatory, antityrosinase and antixanthine oxidase activities of Moroccan propolis”. International Journal of Food Science and Technology 2016, 51, pp. 1762-1773.

M.G. Miguel, S. Aazza, M.D. Antunes, M.L. Faleiro, J.G. Barroso, L.G. Pedro, A.C. Figueiredo, “Mineral and volatile composition of água‑mel from Portugal”. Eur Food Res Technol2016, 242, pp.171-178. DOI 10.1007/s00217-015-2528-5.

N. Bahloul, S.Bellili, S. Aazza, A. Chérif, M.L. Faleiro, M.D. Antunes, M.G. Miguel, W. Mnif, “Aqueous Extracts from Tunisian Diplotaxis: Phenol content, antioxidant and anti-acetylcholinesterase activities, and impact of exposure to simulated gastrointestinal fluids”. Antioxidants 2016, 5, pp. 12.

A.C. Guerreiro, C. M.L. Gago, M.G.C. Miguel, M.L. Faleiro, M.D.C. Antunes, “The influence of edible coatings enriched with citral and eugenol on the raspberry storage ability, nutritional and sensory quality”. Food Packaging and Shelf Life, 2016, 9, pp. 20–28.

J. Melo, P.W. Andrew , M. L. Faleiro (2015) Listeria monocytogenes in cheese and the dairy environment remains a food safety challenge: the role of stress responses. Food Research International 2015, 67, pp.75-90.

A.C Guerreiro, Gago, C., M: L. Faleiro, M. Miguel, M.D.C. Antunes, “The effect of alginate based edible coatings enriched with essential oils constituents on Arbutus unedo L. fresh fruit storage”. Postharvest Biology and Technology, 2015, 100, pp. 226-233.

A.C. Guerreiro, C. Gago, M.L. Faleiro, M. Miguel, M.D.C. Antunes, “Edible coatings enriched with essential oils for extending the shelf-life of ‘Bravo de Esmolfe’ fresh-cut apples”. International Journal of Food Science and Technology, 2015, 51, pp.87-95. doi:10.1111/ijfs.12949.

A.C. Guerreiro, C. Gago, M.L. Faleiro, M. Miguel, M.D.C. Antunes, “Raspberry fresh fruit quality as affected by pectin- and alginate-basededible coatings enriched with essential oils”. Scientia Horticulturae 194, pp.138-146.

Miguel MG, Faleiro ML, Guerreiro AC, Antunes MD (2014) Arbutus unedo L.: chemical and biological properties. Molecules 19: 15799-15823.

Mota L, Figueiredo AC, Pedro LG, Barroso JG, Miguel MG, Faleiro ML, Ascensão, L. (2014) Volatile-Oils composition, and bioactivity of the essential oils of Plectranchus barbatus, P. neochilus, and P. ornatus grown in Portugal. Chemistry & Biodiversity 11: 719-732.

Apolónio J, Faleiro ML, Miguel MG, Neto L (2014) No induction of antimicrobial resistance in Staphylococcus aureus and Listeria monocytogenes during continuous exposure to eugenol and citral. FEMS Microbiology Letters 354: 92-101.

Miguel MG, Antunes MD, Aazza S, Duarte J, Faleiro ML (2013) Honey-based “água-mel” chemical characterization and microbiological quality. Italian Journal of Food Science 25: 275-282.

Miguel, M.G., Faleiro, M.L., Antunes, M.D., Aazza, S., Duarte, J., Silvério, A.R. (2013) Antimicrobial, antiviral and antioxidant activities of “água-mel” from Portugal.  Food and Chemical Toxicology 56: 136-147.

Schrama D, Helliwell N, Neto L, Faleiro ML (2013) Adaptation of Listeria monocytogenes in a simulated cheese medium: Effects on virulence using the Galleria mellonella infection model. Letters in Applied Microbiology 6:421-427.

Melo J, Schrama D, Hussey S, Andrew PA, Faleiro ML (2013) Listeria monocytogenes dairy isolates show a different proteome response to sequential exposure to gastric and intestinal fluids. International Journal of Food Microbiology 163: 51-63.

Melo J, Andrew PA, Faleiro ML (2013) Different assembly of acid and salt tolerance response in two dairy Listeria monocytogenes wild strains. Archives of Microbiology 195: 339-348.

Melo J, Schrama D, Andrew PA, Faleiro ML (2013) Proteomic analysis shows that individual Listeria monocytogenes strains use different strategies in response to gastric stress. Foodborne Pathogens and Disease 10:107-119.



Project Grants


Project Leader

PTDC/AGR-ALI/69751/2006 – “Autolysis in Listeria monocytogenes – what is revealed by proteomic analysis?”

POCI/SAL-IMI/58702/2004 – “A partícula de reconhecimento de sinal da bactéria Streptococcus pneumoniae é essencial para a sua viabilidade”.

QLRT-02219 – “Characterisation of Listeria monocytogenes to provide tools to predict biofilm formation during cheese making”.

POCTI/ESP/42668/2001- “The role of p60 on autolytic behaviour of Listeria monocytogenes”.

Collaborator / Team leader

POCI/AMB/58512/2004 – “Ecotecnologies basead on the use of sulphate reducing bactéria and their apllication on the treatment of acid mine waters”.

POCTI/ESP/41217/2001 – “The incidence of gastric diseases is related with a particular Helicobacter pylori population?”


Representative Images